Ion Exchange Column Chromatography Principle

This separation is done based on the differences in the adsorption coefficient or partition coefficient of the sample with the stationary phase.

Ion exchange column chromatography principle.

Eluent loading sample injection separation of sample elution of analyte a and elution of analyte b shown and explained below. In the stationary phase the analyte is opposite to the charged sites when it passes through the chromatography column. The basic process of chromatography using ion exchange can be represented in 5 steps. Ion exchange chromatography is most often performed in the form of column chromatography.

Elution is the process where the compound of interest is moved through the column. Practical aspects of performing a separation are covered in chapter 2. It works on almost any kind of charged molecule including large proteins small nucleotides and amino acids however ion chromatography must be done in conditions that are one unit away from the isoelectric point of a protein. As you know the chromatography is a process of the separation of molecules from a mixture.

The analytes isolated based on their charge are eluted utilizing a solution of different ionic strength. Ge healthcare s life sciences business offers a wide variety of prepacked columns and ready to use chromatography media. When the mobile phase along with the mixture that needs to be separated is introduced from the top of the column. Principles of ion exchange this chapter provides a general introduction to the theoretical principles that underlie every ion exchange separation.

An understanding of these principles will enable the separation power of ion exchange chromatography iex to be fully appreciated. Ge healthcare ion exchange chromatography principles and methods 11000421 ge healthcare life sciences mononuclear cells isolation of methodology and applications. Ion exchange chromatography is an interesting type of column chromatography. Ion exchange column chromatography a chromatography technique in which the stationary phase is always ion exchange resin.

Working principle of ion exchange chromatography. The samples with charged analytes will be used as a liquid phase. However there are also thin layer chromatographic methods that work basically based on the principle of ion exchange. Deae cellulose is an anionic exchanger cm cellulose is a cationic exchanger.

Ion exchange separations are carried out mainly in columns packed with an ion exchanger. Table of contents 1 definition 2 types of ic 3 history 4 principle 5 basic process of ic 6 stationary phase or ion exchange materials used in ic 7 detection methods 8 chromatogram 9 basic instrumentation 10 conditions to be remembered in ic 11 applicaions of ic principles of ion exchange chromatography the most popular method for the purification of proteins and other charged molecules is ion. They are made up of styrene and divinylbenzene.