Ion Exchange Column Set Up

Padding for the column on xs screens and up ion grid columns.

Ion exchange column set up.

An ion exchange process is to be used to soften water at the rate of 500 gpm. A synthetic zeolite resin will be packed in shells with diameter of 5 ft. If this type of simple equipment is used then a nylon cloth over the surface of each. The hardness ions ca and mg move into the resin beads and each of these divalent.

Mount the column top piece and connect to a pump. A glass column with a sintered glass bottom is ideal but otherwise a column with a rubber stopper at each end with a glass tube through each stopper will work. The sample ion can exchange with the counterion to form an ion pair with the matrix. The retention of the sample ion is based on the affinity of the different ions for the site on the matrix and on a number of other solution parameters such as ph ionic strength counterion type etc.

Padding for the column on sm screens and up ion grid column padding xl. Column cleaning for ion exchange chromatography and chromatofocusing appendix 10 extracted from ion exchange chromatography chromatofocusing ge healthcare 2007 correct preparation of samples and buffers and application of a high salt wash 1 m nacl at the end of each separation should keep most columns in good condition. The presence of a sample ion of the same ionic charge as the counterion sets up another equilibrium. Padding for the column on xl screens and up ion grid column padding xs.

This video demonstrates the use of a cation exchange column to convert a salt to an acid. Padding for the column on md screens and up ion grid column padding sm. Column size most industrial ion exchange columns have a diameter between 0 5 and 3 2 m. The number of total columns in the grid.

The raw water has total hardness of 100 mg l as caco 3. The resin has an exchange capacity of 320 kilograins of caco 3 per ft when regenerated at the 3rate of 15 ib of salt per ft. To carry out initial trials a 25mm diameter glass or sometimes a plastic column is suitable. When slurry volume is greater than the total volume of the column connect a second glass column to act as a reservoir see ordering information for details.

The basics of ion exchange chromatography published january 24 2017 highly pure proteins are vital for successful experiments. They play roles in research as assay reagents especially for spr applications therapeutic candidates and of course as the subjects of structural and biochemical studies. Rohm and haas ion exchange ion exchange introduction 4 fd sep 2008 to soften water you take a cation exchange resin on which the mobile ion inside the beads is sodium na and you pass the hard water through a column filled with the sodium form resin.