Ion Exchange Column Hplc

In cation exchange the analyte is positively charged a cation while the chromatographic material is negatively charged an anion.

Ion exchange column hplc.

Exchange capacity is dependent upon the ph of the mobile phase and in anion exchange chromatography. C18 and ion exchange have very different adsorption retention mechanisms. Skip to content. C8 hplc columns are used when less retention compared to a c18 is needed.

Hawach scx hplc column with excellent peak shape separation efficiency stability and reproducibility. As mobile phase acidity decreases ph increases the exchange capacity decreases. Ion exchange columns that run on hplc systems do exist. Is a kind of high efficiency ion exchange hplc column.

I ll assume that you know about the ion exchange hplc columns. Ion exchange chromatography separates molecules based on differences in the net charge of the molecules. C18 hplc columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage. Propac wcx 10 carboxylate ion exchange hplc column 10 μm 4 mm x 50 mm thermo scientific use thermo scientific propac wcx 10 lc columns to provide exceptionally high resolution for separations of proteins and their variants.

Cation and anion exchange columns polymethacrylate based biosuite ion exchange iex columns include four separation chemistries sp strong and cm weak cation exchangers and q strong and deae weak anion exchangers bonded to a ph stable ph 2 12 methacrylic ester based polymeric resin. C4 and c5 hplc columns are commonly used to separate large macromolecules such as proteins. Cation exchange hplc columns. Anion exchange capacity is a measurement of the number of negative charges anions that the exchange resin can bind to and is reported in singly charged ion equivalents per 1 gram of resin.

It is usually used for protein purification but may be used for purification of oligonucleotides peptides or other charged molecules. Biosuite ion exchange hplc columns. Ion exchange chromatography separates molecules based on differences between the overall charge of the proteins. Also supply other types of hpcl columns.

C18 and ion exchange have very different adsorption retention mechanisms. It s a typical cation exchange column using a silica gel matrix with an aromatic sulfonic acid group as the bonding phase. Ion exchange columns that run on hplc systems do exist. The cl ion of the hcl would exchange with the hydroxide converting this into h and oh which is non conducting water.