Ion Exchange Column Ge Healthcare

Ion exchange chromatography columns and resins.

Ion exchange column ge healthcare.

The supernatant containing the heat stable cam was loaded onto a resource q anion exchange column ge healthcare equilibrated in buffer a. Cytiva formerly ge healthcare life sciences hitrap iex hp prepacked columns. Principle of ion exchange chromatography ion exchange iex chromatography can separate molecules or groups of molecules that have only slight differences in charge. 3 protein mix purified at 1 ml min and containing.

Use for high resolution small scale protein purification. Ge healthcare ion exchange columns and media selection guide imagination at work. Ge healthcare ion exchange chromatography principles and methods 11000421 ge healthcare life sciences mononuclear cells isolation of methodology and applications isolation of mononuclear cells methodology and applications. But remember that the ion exchange capacity of a weak ion exchanger varies with ph.

Ion exchange chromatography iex the most potent fraction f6 from rp hplc was loaded onto a hitrap sp hp cation exchange chromatography column 34 µm. For in depth information about iex download our iex handbook. Ion exchange chromatography resins can be used at high flow rates because binding kinetics for iex are fast and rigid chromatography particles can be used. Ge healthcare bio sciences ab björkgatan 30 751 84 uppsala sweden ion exchange chromatography chromatofocusing principles and methods ion exchange chromatography chromatofocusing principles and methods imagination at work 11000421aa ion cover indd 2 2010 04 01 12 12 53.

Cytiva life sciences hitrap iex hp prepacked columns are prepacked ready to use sepharose high performance strong cation exchange columns. Shop a large selection of protein purification products and learn more about cytiva formerly ge healthcare life sciences sephadex ion exchange media qae sephadex a 50. Column cleaning for ion exchange chromatography and chromatofocusing appendix 10 extracted from ion exchange chromatography chromatofocusing ge healthcare 2007 correct preparation of samples and buffers and application of a high salt wash 1 m nacl at the end of each separation should keep most columns in good condition. Separation is based on the reversible interaction.

How does ion exchange chromatography work. The net surface charge of proteins varies according to the surrounding ph. Column packing and preparation.